A) Different acceptor blocks can be used for the generation of tissue

Download scientific diagram | (A) Different acceptor blocks can be used for the generation of tissue microarrays. Whereas conventional histological cassettes (left and middle) are routinely used, individually formed paraffin wax blocks are superior if 2 mm punches are required. (B) Typical examples of tissue arrays. On the left, 0.6 mm punches have been used, whereas on the right a block with cores of 2 mm diameter is shown. (C) Problems of TMA blocks. A broken array is shown on the left. The array was composed of 2 mm diameter punches using a conventional cassette and the tension within the paraffin wax was too high. On the right side, the acceptor block shows a thin white line, which should be regarded as a first warning sign of increased tension within the paraffin wax, which might lead to array breakage. (D) Haematoxylin and eosin stained sections of liver tissue (2 mm diameter punches on the left) and cancer tissue (0.6 mm on the right) from publication: Demystified Tissue microarray technology | Several "high throughput methods" have been introduced into research and routine laboratories during the past decade. Providing a new approach to the analysis of genomic alterations and RNA or protein expression patterns, these new techniques generate a plethora of new data | Tissue Microarrays, Oligonucleotide Array Sequence Analysis and TMA | ResearchGate, the professional network for scientists.

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General procedure. (A) Microarray generation with laser induced

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